Mycobacterium leprae , Rickettsia , Chlamydia , and Treponema pallidum are obligate parasites. Bacterial culture media can be classified on the basis of composition, consistency, and purpose. Solid medium contains agar at a concentration of 1. Solid medium has a physical structure and allows bacteria to grow in physically informative or useful ways e.
Solid medium is useful for isolating bacteria or for determining the colony characteristics of the isolate. Semisolid medium is prepared with agar at concentrations of 0. Semisolid medium has a soft custard-like consistency and is useful for the cultivation of microaerophilic bacteria or for the determination of bacterial motility.
Broth medium serves various purposes such as propagation of a large number of organisms, fermentation studies, and various other tests. Many special-purpose media are needed to facilitate recognition, enumeration, and isolation of certain types of bacteria.
To meet these needs, numerous media are available. Basal media also called general-purpose media are basically simple media that support the growth of most non-fastidious bacteria.
Peptone water, nutrient broth, and nutrient agar NA are considered basal mediums. These media are generally used for the primary isolation of microorganisms. Addition of extra nutrients in the form of blood, serum, egg yolk, etc, to the basal medium makes an enriched medium.
The product portfolio. To make sure the latest. Solid culture medium used in the assays of Colistimethate and Polymicin by the.. Where a product trademark, registration mark, or other protected mark is made in.
Types of Culture Media used in Pharmaceutical Microbiology. Associate Prof, Microbiology. A pure culture. Contaminated culture contains unwanted species of organisms. Peptone: Spray dried hydrolysate of various proteins. Used as solidifying agent for culture media in petri plates.. Types of culture media used in microbiology. Media are of different types on consistency and chemical composition. On Consistency: 1. Types of culture media 1. Types of culture media Prepared by Samira fattah Assis.
College of health sciences-HMU Lab 5 2. Uses of culture medium It is important to grow microorganisms outside the body for the following purposes: 1. Types of culture media I. Classification based on physical state a solid medium b semi solid medium c liquid medium 6.
Classification based on the ingredients a simple medium b complex medium c synthetic or defined medium d Special media 7. Classification based on physical state Solid medium agar is the most commonly used solidifying agent. Nutrient broth Classification based on the ingredients Simple media - eg: Nutrient broth, N.
Complex media such as blood agar, it has ingredients that exact components are difficult to estimate. Chocolate agar contain red blood cells that have been lysed by slowly heating to 80 c. CLED, serratiadfd Media Preparation Assemble all of your chemicals in your work area before you begin. Accurately weigh each of the dry ingredients in your culture media.
Add each dry culture medium ingredient into a flask. Add distilled water to make the correct volume. Heat AND stir agar will burn if it is not stirred until all of the ingredients go into solution. When the media boils, it is ready for sterilization. Nutrient broth has the following composition: Peptone 10 g Meat extract 10 g NaCl 5g Water ml NB is the basis of many common media. Types of culture media I. Based on their consistency a solid medium b liquid medium c semi solid medium II.
Liquid media — no agar. Semi solid medium — Nutrient broth containing 0. In other words, a fastidious organism will only grow when specific nutrients are included in its diet.
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